A comparative study of transfection of rat mesenchymal stem cells using polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles and lipofectamine
نویسندگان
چکیده
Stem cell transfection agents have traditionally included lipofectamine, but newer agents include polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles. This study was done to compare these two methods in transfecting cultured ratmesenchymal stem cells. Magnetic ferro-ferric oxide nanoparticles were coated with polyethyleneimine using co-precipitation of alkali metal ions, forming a mobility diameter of approximately 50 nm. It dispersed well in water. Compared to the lipofectamine, polyethyleneimine-coated magnetic ferro-ferric oxide nanoparticles had higher transfection rate-in rat mesenchymal stem cells (P<0.05). Meanwhile, cell proliferation analysis revealed less toxicity of such nanoparticles to rat mesenchymal stem cells. In summary, polyethyleneiminecoated magnetic ferro-ferric oxide nanoparticles are safe and had a higher transfection rate in cultured ratmesenchymal stem cells, indicating that this improved technique of mesenchymal stem cell transfection may have clinical application potential.
منابع مشابه
In vitro Labeling of Neural Stem Cells with Poly-L-Lysine Coated Super Paramagnetic Nanoparticles for Green Fluorescent Protein Transfection
Background: The magnetic nanoparticle-based transfection method is a relatively new technique for delivery of functional genes to target tissues. We aimed to evaluate the transfection efficiency of rat neural stem cell (NSC) using poly-L-lysine hydrobromide (PLL)-coated super paramagnetic iron oxide nanoparticles (SPION). Methods: The SPION was prepared and coated with PLL as transfection agent...
متن کاملEfficient delivery of C/EBP beta gene into human mesenchymal stem cells via polyethylenimine-coated gold nanoparticles enhances adipogenic differentiation
The controlled differentiation of stem cells via the delivery of specific genes encoding appropriate differentiation factors may provide useful models for regenerative medicine and aid in developing therapies for human patients. However, the majority of non-viral vectors are not efficient enough to manipulate difficult-to-transfect adult human stem cells in vitro. Herein, we report the first us...
متن کاملInnovative Strategy for MicroRNA Delivery in Human Mesenchymal Stem Cells via Magnetic Nanoparticles
Bone marrow derived human mesenchymal stem cells (hMSCs) show promising potential in regeneration of defective tissue. Recently, gene silencing strategies using microRNAs (miR) emerged with the aim to expand the therapeutic potential of hMSCs. However, researchers are still searching for effective miR delivery methods for clinical applications. Therefore, we aimed to develop a technique to effi...
متن کاملInsights into the mechanism of magnetic particle assisted gene delivery.
In magnetic particle assisted gene delivery DNA is complexed with polymer-coated aggregated magnetic nanoparticles (AMNPs) to effect transfection. In vitro studies based on COS-7 cells were carried out using pEGFP-N1 and pMIR-REPORT-complexed, polyethylenimine (PEI)-coated iron oxide magnetic nanoparticles (MNPs). PEI-coated AMNPs (PEI-AMNPs) with average individual particle diameters of 8, 16 ...
متن کاملEfficient gene delivery to human umbilical cord mesenchymal stem cells by cationized Porphyra yezoensis polysaccharide nanoparticles
This study centered on an innovative application of Porphyra yezoensis polysaccharide (PPS) with cationic modification as a safe and efficient nonviral gene vector to deliver a plasmid encoding human Wnt3a (pWnt3a) into human umbilical cord mesenchymal stem cells (HUMSCs). After modification with branched low-molecular-weight (1,200 Da) polyethylenimine, the cationized PPS (CPPS) was combined w...
متن کامل